ScienCell's Human ELOVL2 Promoter and Global DNA Dual Methylation Quantification qPCR Assay Kit (HEGDD) is designed to simultaneously quantify the level of human ELOVL2 promoter and LINE1 promoter methylation. The reference DNA sample consists of a 1:1 ratio of methylated ELOVL2 promoter copies to non-methylated ELOVL2 promotor copies (both copies represent bisulfite converted promoter sequences) and a 1:1 ratio of methylated LINE1 promoter copies to non-methylated LINE1 promotor copies (both copies represent bisulfite converted promoter sequences), and serves as a reference for calculating the ratio of methylated to non-methylated ELOVL2 promotor and LINE1 promoter of target samples. The carefully designed primers ensure: (i) high efficiency for trustworthy quantification; and (ii) negligible non-specific amplification. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis for amplification specificity and by template serial dilution for amplification efficiency.