Product name
Collagen I Cell Culture Surface Coating Kit (CCCSCK)
Catalog number
SC8188
Unit size
10 mg
Type I collagen, a fibrous protein abundant in connective tissues is the major component and the primary determinant of tensile strength of the extracellular matrix (ECM).
Type I collagen is widely used as a thin layer on tissue-culture surfaces to enhance the attachment and proliferation of a variety of cells including endothelial cells, fibroblasts, hepatocytes, and epithelial cells.
In addition, collagen I can self-assemble into a 3-D supramolecular gel in vitro, making it an ideal biological scaffold to promote more in vivo-like cellular morphology and functions.
The Collagen I-Cell Culture Surface Coating Kit includes collagen I purified from rat tail tendon by modification of the Bell Method and supplied as a sterile liquid in 1/1000 acetic acid [1].
The kit also includes a 100X collagen I solvent, which can be used to dilute collagen I to the appropriate concentration for the coating of cell culture vessels.
Quality Control
CCCSCK is tested for the adherence of cells to the culture dish and promotion of cell growth.
CCCSCK is negative for bacterial contamination.
Product Use
CCCSCK is used to coat cell culture vessels in vitro.
CCCSCK is for research use only. It is not approved for human or animal use, or application in clinical or in vitro diagnostic procedures.
Shipping
All components are shipped on gel ice.
References
[1] Bell E, Ivarsson B, Merrill C. (1979) "Production of a tissue-like structure by contraction of collagen lattices by human fibroblasts of different proliferative potential in vitro." Proc Natl Acad Sci USA, 76(3): 1274-1278.