Quality Control: The specificity of the primer sets is validated by qPCR with melt curve analysis. The PCR products are analyzed by gel electrophoresis. The efficiency of the mtDNA primer sets is validated by template serial dilution (See Appendices 1). The copy number of reference mtDNA sample per μL is determined by the qPCR standard curve method.
Product Use: SC8968 is for research use only. It is not approved for human or animal use, or for application in clinical or in vitro diagnostic procedures.
Shipping and Storage: The product is shipped on dry ice. Upon receipt, store the GoldNStart TaqGreen qPCR master mix (Cat. no. MB6018a-1) in the dark at -20°C in a manual defrost freezer, the primers (Cat. no. SC8968a) and the reference genomic DNA sample (Cat. no. SC8968c) at -20°C in a manual defrost freezer, and the nuclease-free H2O (Cat. no. SC8968b) at +4°C. Once thawed, do NOT refreeze GoldNStart TaqGreen qPCR master mix (Cat. no. SCMB6018a-1), and keep in the dark at +4°C or on ice at all times.
References: [1] Picard M. Blood mitochondrial DNA copy number: What are we counting?. Mitochondrion. 2021 Sep 1;60:1-1.
[2] Rosa HS, Ajaz S, Gnudi L, Malik AN. A case for measuring both cellular and cell‐free mitochondrial DNA as a disease biomarker in human blood. The FASEB Journal. 2020 Sep;34(9):12278-8)