DNA dA-Tailing Kit, (25 Reactions)
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DNA dA-Tailing Kit, (25 Reactions)

DNA modification

The DNA dA-Tailing Kit efficiently adds a non-template dAMP (dA) to the 3’ end of a blunt-ended DNA fragment. This incorporated 3’-dA prevents concatemer formation and prepares the DNA fragment for subsequent ligation of adaptors or cloning vectors that have complementary 3’-dT overhangs. The Klenow Fragment (3′→ 5′ Exo-) in this kit adds the 3’-dA via its DNA polymerase activity. This enzyme lacks 5’→3’ exonuclease activity and has mutations that effectively abolish the inherent 3’→5’ exonuclease activity, thus preventing degradation of this 3’-dA. This kit has been optimized to prepare compatible overhangs for the next step of DNA sample preparation for next generation sequencing. The Klenow Fragment (3′→ 5′ Exo-) enzyme is also available as a stand-alone enzyme (Cat. No. E038).

Catalog number: ABME009
Article number:ABME009
Unit:pcs
Stock status:Special Order Item
276,00€ / pcs
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