HNPC are isolated from the nucleus pulposus of human intervertebral disc. HNPC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HNPC are characterized by immunofluorescence with antibodies specific to fibronectin and vimentin. HNPC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HNPC are guaranteed to further expand for 15 population doublings under the conditions provided.
Recommended Medium: It is recommended to use Nucleus Pulposus Cell Medium (NPCM, Cat. no. SC4801) for the culturing of HNPC in vitro.
Product Use: HNPC are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage: Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping: Dry ice.