HO-f are isolated from human femur. HO-f are cryopreserved at P0 and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HO-f are characterized by the cytochemical detection of AP and mineral deposition. HO-f are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HO-f are guaranteed for 15 population doublings under the conditions provided.
Recommended Medium: It is recommended to use Osteoblast Medium (OsM, Cat. no. SC4601) for the culturing of HO-f in vitro.
Product Use: HO-f are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage: Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping: Dry ice.