Pulmonary alveolar epithelial cells (PAEpiC), comprised of alveolar type I and type II epithelial cells, line more than 99% of the internal surface area of the lung. Type I cells are large squamous cells whose thin cytoplasmic extensions cover >95% of the internal surface area. They contain aquaporins and exhibit the highest osmotic water permeability of any mammalian cell type. Type II cells, which cover 2-5% of the surface area, produce, secrete, and recycle pulmonary surfactant. Type II cells contain Na+-, K+-ATPase and amiloride-sensitive epithelial Na+ channels.
Human Pulmonary Alveolar Epithelial Cells (HPAEpiC) are isolated from human lung tissue and cryopreserved at Passage 1 and delivered frozen.
Each vial contains 1 million cells in 1 ml volume.
HPAEpiC are characterized by immunofluorescence with antibodies specific to CK-18, CK-19, and vimentin.
HPAEpiC are tested negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi.
HPAEpiC are guaranteed to further culture under the conditions provided; however, HPAEpiC are not recommended for expanding or long-term cultures since the cells would differentiate to become type I alveolar epithelial cells immediately after plating and type I alveolar epithelial cells do not proliferate in culture.
Recommended Medium
It is recommended to use Alveolar Epithelial Cell Medium (AEpiCM, Cat. no. SC3201) for culturing HPAEpiC in vitro.
Product Use
HPAEpiC are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping
Dry ice.