HOSEpiC are isolated from human ovary. HOSEpiC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HOSEpiC are characterized by immunofluorescence with antibodies specific to cytokeratine-14, -18, and -19. HOSEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HOSEpiC are guaranteed to further expand for 5 population doublings under the conditions provided.
Recommended Medium: It is recommended to use Ovarian Epithelial Cell Medium (OEpiCM, Cat. No. SC7311) for expanding HOEpiC in vitro.
Product Use: HOEpiC are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage: Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Shipping: Dry ice.
Reference: [1] Murdoch WJ. (1995) Endothelial cell death in preovulatory ovine follicles: possible implication in the biomechanics of rupture. J Reprod Fertil 1995; 105:161–164.
[2] Katabuchi H, Okamura H. (2003) Cell biology of human ovarian surface epithelial cells and ovarian carcinogenesis. Medical Electron Microscopy 36(2):74-86.