HOrF from ScienCell Research Laboratories are isolated from human oral tissue. HOrF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HOrF are characterized by their spindle morphology and immunofluorescence with antibodies specific to fibronectin. HOrF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HOrF are guaranteed to further expand for 15 population doublings under the conditions provided.
Recommended Medium: It is recommended to use Fibroblast Medium (FM, Cat. no. SC2301) for the culturing of HOrF in vitro.
Product Use: HOrF are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage: Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping: Dry ice.
References: [1] Stephens P, Davies K, Occleston N, Pleass R, Kon C, Daniels J, Khaw P, Thomas D. (2001) “Skin and oral fibroblasts exhibit phenotypic differences in extracellular matrix reorganization and matrix metalloproteinase activity.” Br J Dermatol. 144(2): 229-237.
[2] Enoch S, Wall I, Peake M, Davies L, Farrier J, Giles P, Baird D, Kipling D, Price P, Moseley R, Thomas D, Stephens P. (2009) “Increased oral fibroblast lifespan is telomerase-independent.” J Dent Res. 88(10): 916-921.
[3]Thode C, Jørgensen TG, Dabelsteen E, Mackenzie I, Dabelsteen S. (2011) “Significance of myofibroblasts in oral squamous cell carcinoma.” J Oral Pathol Me. 40(3): 201-207.