The mammalian lens consists of two types of cells, 1) lens fiber cells which form the bulk of the lens, 2) a monolayer of epithelial cells that cover the anterior surface of the fibers.
Lens epithelial cells are responsible for homeostasis regulation of the lens including electrolyte and fluid transport.
During the development, lens epithelial cells progressively differentiate and migrate from the equatorial region into the interior of the lens to produce transparent crystallins, elongate to form lens fiber cells. Lens epithelial cell differentiation and lens polarization are regulated by growth factors present in the ocular fluids.
Human Lens Epithelial Cells-adult (HLEpiC) are isolated from human lens and are cryopreserved at P0 and delivered frozen.
Each vial contains 500 000 cells in 1 ml volume.
Human Lens Epithelial Cells-adult (HLEpiC) are characterized by immunofluorescence with antibodies specific to cytokeratin-18, cytokeratin-19- 19, and fibronectin.
HLEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi.
HLEpiC are guaranteed to further expand for 10 population doublings under the culture condition provided.
Recommended Medium
It is recommended to use Epithelial Cell Medium (EpiCM, Cat. no. SC4101) for culturing HLEpiC in vitro.
Product Use
HLEpiC are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen, and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping
Dry ice.