The prostate is a organ of the male mammalian reproductive system and is structural and functional controlled by androgens. The prostate is composed of three compartments, which can be identified by the cytokeratin expression profile. The basal cells express predominantly high molecular weight cytokeratins (CK5 and CK14), the luminal secretory cells express predominantly low molecular weight cytokeratins (CK8 and CK18) and the intermediate cells express a combination of basal and luminal cytokeratins. Prostate epithelial cell (PrEpiC) culture provides unique cell-model to study many important features of the prostate, as well as, chemical and hormonal carcinogenesis.
Human Primary Prostate Epithelial Cells (HPrEpiC) are isolated from normal human prostate tissue.
HPrEpiC are cryopreserved at passage 1 and delivered frozen.
Each vial contains 500 000 in 1 ml volume.
HPrEpiC are characterized by immunofluorescence with antibodies specific to cytokeratine (CK-14, CK-18 and CK-19).
HPrEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi.
HPrEpiC are guaranteed to further expand for 15 population doublings under the conditions provided.
Recommended Medium
It is recommended to use Prostate Epithelial Cell Medium (PEpiCM, Cat. no. SC4411) for culturing HPrEpiC in vitro.
Product Use
HPrEpiC are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping
Dry ice.