Human Leydig Cells (HLC) are located in the interstitium of the testis near the seminiferous tubules. One of the important functions of LC is to produce testosterone after stimulation by pituitary luteinizing hormone. Cultured HLC are a useful in vitro model to better understand testicular development and to develop treatments for male reproductive disorders.
Human Leydig Cells are isolated from human testis. HLC are cryopreserved at passage 1 and delivered frozen. Each vial contains 500000 cells in 1 ml volume.
HLC are characterized by immunofluorescence with antibody specific to GATA-4 and CK-18.
HLC are tested and found negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi.
HLC are guaranteed to further expand for 5 population doublings under the conditions provided. however, HLC are not recommended for expanding or longterm cultures due to limited expansion capacity.
Recommended Medium
It is recommended to use Leydig Cell Medium (LCM, Cat. no. SC4511) for the culturing of HLC in vitro.
Product Use
HLC are for in vitro laboratory research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping
Dry ice.