HLMC from ScienCell Research Laboratories are isolated from human liver. HLMC are depleted of erythrocytes and hepatic macrophages, cryopreserved immediately after isolation,and delivered frozen. HLMC are a mixed population of cells that include T-lymphocytes, B-lymphocytes, NK-cells, and monocytes. Each vial contains at least 10 million cells in 1 ml volume. HLMC are quality control tested for viability. HLMC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HLMC can be maintained for a short period of time in culture using the conditions provided by ScienCell Research Laboratories and are not intended for long-term culture.
Recommended Medium: It is recommended to use HematoGro Medium (HeGM, Cat. no. SC5501) for short-term maintenance of HLMC in vitro.
Product Use: HLMC are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage: Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping: Dry ice.
References: [1] Hata K, Zhang X, Iwatsuki S, Van Thiel D, Herberman R, Whiteside T. (1990) "Isolation, phenotyping, and functional analysis of lymphocytes from human liver." Clin Immunol Immunopathol. 56(3): 401-419.