Human Pancreatic microvascular endothelial cells (HPaMEC) are isolated from human pancreas. HPaMEC are cryopreserved at passage one and each vial contains >500 000 cells in 1 ml volume.
Cell Characterization:
HPaMEC are characterized by immunofluorescence with antibodies specific to vWF/Factor VIII and CD31 (PECAM). HPaMEC are tested negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HPaMEC are guaranteed to further expand for 10 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium:
It is recommended to use Endothelial Cell Medium (ECM, Cat. no. SC1001) for culturing HPaMEC in vitro.
Shipping Condition:
Dry ice.
Storage Condition:
In liquid nitrogen
Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Product Usage:
HPaMEC are for in vitro laboratory research use only. They are not approved for use in humans or animals, or for application in in vitro diagnostic procedures.
References:
[1] Zanone M, Favaro E, Camussi G. (2008) "From endothelial to beta cells: insights into pancreatic islet microendothelium."Curr Diabetes Rev. 4(1): 1-9.
[2] Konstantinova I and Lammert E. (2004) "Microvascular development: learning from pancreatic islets." Bioessays 26(10): 1069-1075.
[3] Nielsen M, Mortensen M, DetlefesenS. (2016) "Key players in pancreatic cancer stroma interaction: cancer-associated fibroblasts, endothelial and inflammatory cells. World J Gastroenterol 22(9): 2678-2700.