HMF are isolated from human breast. HMF are cryopreserved at P0 and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HMF are characterized by immunofluorescence with antibodies specific to fibronectin, CD90, and vimentin. HMF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HMF are guaranteed to further expand for 15 population doublings under the conditions provided.
Recommended Medium: It is recommended to use Fibroblast Medium (FM, Cat. No. SC2301) for the culturing of HMF in vitro.
Product Use: HMF are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage: Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture is needed for experiments.
Shipping: Dry ice.
Reference: [1] D Zangani, KM Darcy, PA Masso-Welch, ES Bellamy, MS Desole, MM Ip (1999) Multiple differentiation pathways of rat mammary stromal cells in vitro: acquisition of a fibroblast, adipocyte or endothelial phenotype is dependent on hormonal and extracellular matrix stimulation. Differentiation. 64(2)91-101.
[2] WG Jiang, D Grimshaw, TA Martin, G Davies, C Parr, G Watkins, J Lane, R Abounader, J Laterra and RE Mansel (2003) Reduction of Stromal Fibroblast-induced Mammary Tumor Growth, by Retroviral Ribozyme Transgenes to Hepatocyte Growth Factor/Scatter Factor and its Receptor, c-MET. Clinical Cancer Research (9)4274-4281.