Name: MesenFectagen
SKU: SC0933
Price: 660.00 EUR
Availability: InStock

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Cell Transfection

The delivery of foreign DNA into eukaryotic cells is one of the most common molecular biology techniques to study biological mechanisms. However, unlike transformed cell lines, the efficient transfection of primary cells can be a problem. MesenFectagen is a cationic polymer-based transfection system specifically designed and optimized for efficient transfection of primary mesenchymal stem cells. Transfection with MesenFectagen can be carried out in the presence of antibiotics and serum. Instead of normal two-day transfection, an optimized one-day transfection procedure can be performed for time-saving and highly reproducible transfection. One ml of MesenFectagen reagent is sufficient for up to 250 transfections per well in 96-well plate. (SC0933)

Article number:SC0933

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Description

Storage/Handling: Upon receipt, aliquot and store MesenFectagen reagent A at -20°C, avoid repeated freezing/thawing cycles. Once thawed, store MesenFectagen reagent A at 4°C and use in a month. MesenFectagen reagent B can be keptat 4°C.

Quality Control: Each lot of MesenFectagen is performance tested by transfecting Human Mesenchymal Stem Cells (HMSCs,Cat. No. SC7500, ScienCell™) with Promega® ρSV-bata-Galactosidase control vector. Gene expression is assayedby X-gal staining 24 hours post transfection. Typically, 20-35% transfection efficiency can be achieved.