Storage/Handling: Upon receipt, aliquot and store FibroFectagen reagent A at -20°C, avoid repeated freezing/thawing cycles. Once thawed, store FibroFectagen reagent A at 4°C and use in a month. FibroFectagen reagent B can be kept at4°C.
Quality Control: Each lot of FibroFectagen is performance tested by transfecting Human Pulmonary Fibroblasts (HPFs, Cat.No. SC3300, ScienCell™) with Promega® ρSV-bata-Galactosidase control vector. Gene expression is assayed by X-gal staining 24 hours post transfection. Typically, 40-70% transfection efficiency can be achieved.