EndoFectagen is a cationic polymer-based transfection system specifically designed and optimized for efficient transfection of primary endothelial cells. Transfection with EndoFectagen can be carried out in the presence of antibiotics and serum. Instead of normal two-day transfection, an optimized one-day transfection procedure can be performed for time-saving and highly reproducible transfection. One ml of EndoFectagen reagent is sufficient for up to 250 transfections per well in 96-well plate.
Storage/Handling: Upon receipt, aliquot and store EndoFectagen reagent A at -20°C, avoid repeated freezing/thawing cycles. Once thawed, store EndoFectagen reagent A at 4°C and use in a month. EndoFectagen reagent B can be kept at 4°C.
Quality Control: Each lot of EndoFectagen is performance tested by transfecting Human Umbilical Vessel Endothelial Cells (HUVECs, Cat. No. SC8000) with Promega® ρSV-bata-Galactosidase control vector. Gene expression is assayed by X-gal staining 24 hours post transfection. Typically, 30-60 % transfection efficiency can be achieved.