The PC3 prostate cancer cell line is widely used to represent a highly aggressive form of prostate cancer. The GRPR-PC3-Luciferase Stable Cell Line has been stably transfected with pcDNA3.1/hygro/Luc705, resulting in the insertion of the Luc705 cassette. The Luc705 cassette is a Firefly Luciferase coding sequence containing an aberrant intron; which prevents the correct expression of the Luciferase gene. Removal of the aberrant by various mechanisms - such as a splice shifting oligonucleotide (Oligonucleotide Treatment) - allows for proper translation of the gene, resulting in bioluminescence of the cell. The GRPR-PC3-Luciferase Stable Cell Line is useful for studying intracellular delivery of oligonucleotides and oligonucleotdie-conjugates via receptor mediated targeting. Quantity: 10⁶ cells/1.0 mlOrganism: HumanSource Organ: prostateBioSafety Level: IIGrowth Properties: AdherentMorphology: Epithelial-likeSeeding Density: Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.Applications: For Research Use OnlyPropagation: The base medium for this cell line is Prigrow IV medium available at abm, Cat. No. TM004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999) to a final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: +37.0°C.Quality Control: 1) Measured cellular uptake of peptide through flow optometry analysis (Figure 2A). 2) Analysed luciferase activity of peptide compared to controls via RT-PCR (Figure 2B&C). Shipping Conditions: Dry IceStorage Conditions: -180°C