This colorimetric assay is based on the reaction between 5’, 5’-Dithiobis 2-nitrobenzoic acid (DTNB) and GSH to form TNB, which exhibits maximum absorbance at 412 nm. The intensity of the absorbance is proportional to the GSH level in the sample. The GSH/GSSG Ratio Assay kit measures the ratio between GSH and GSSG in different types of samples, such as tissues, cell lysate and plasma.
Product Use: This product is for research purposes only and not for use in animals, humans, or diagnostic procedures.
Quality Control: Data from the GSH/GSSG Ratio Assay kit measuring GSSG solutions with concentrations ranging from 0.195 to 6.25 μM show a linear relationship between OD412 nm and GSSG concentration (Figure 1). Linear detection range is 0.05 μM to 6.25 μM in 96-well plate assay.
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