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Cell based assay
The reduction of tetrazolim salts to colored formazan compounds by succinate-tetrazolim reductase, which exists in viable cells, provides a sensitive and accurate method to measure cell viability and proliferation. The most commonly used tetrazolim salt, MTT [3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide], however, suffers from the disadvantage that the formazan dye produced from MTT is extremely water insoluble, so an additional extraction step is needed for spectrophotometric quantification. Instead of MTT, the ScienCell™ WST-1 Cell Viability & Proliferation Assay utilizes a tetrazolim salt WST-1[2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium]. WST-1 produces a highly water soluble formazan upon metabolically active cells, allowing a direct and user-friendly colorimetric measurement of cell viability and proliferation. (SC8038)
Article number:SC8038
Unit:
Stock status:Special Order Item
Quality Control: Human Astrocytes (Cat. No. SC1800, ScienCell) serial diluted are plated in 96-well plate. The WST-1 Cell Viability & Proliferation Assay is applied and a linear relationship can be observed between signal produced (OD450nm-OD630nm) and thenumber of cells.